Colony Morphology of Yeast Isolates from Tuak and Its Application in Producing Ethanol From Sugarcane Bagasse

Authors

  • Hermansyah Hermansyah Chemistry Dept Faculty of Mathematics and Natural Sciences Sriwijaya University Indonesia
  • Feggy Arini
  • Nirwan Syarif
  • Heni Yohandini

Abstract

Abstract: Colony morphology yeast isolates from tuak and its application in producing ethanol from sugarcane bagasse has been conducted. Yeast isolates used in this work were HT4, HT5, and HT20 obtained from Tuak. These isolates have cream to yellow in color, spherical to oval in shape, and grow as budding cells. Our goal is to find yeast isolates used as microbial agent in fermentation process producing ethanol from lignocellulosic biomass as raw material.  Based upon morphology showed that these isolates were Candida species.  Isolate HT5 then used to see its potent  as  agent in bioethanol production.  Fermentation of 5 g sugarcane bagasse substrate resulted 0.0008% ethanol.  This results indicated that isolate has a potent as microbial agent for fermentation, however the optimum condition of process is needed to furthermore study.    

Keywords: Candida species, bioethanol, Tuak

Abstrak (Indonesian): Telah dilakukan penelitian terhadap morfologi koloni dari isolat khamir dari tuak dan aplikasinya dalam produksi etanol dari ampas tebu.  Isolat khamir yang digunakan adalah koloni HT4, HT5, dan HT20  yang telah didapat dari tuak.  Isolat-isolat ini memiliki bentuk warna krem hingga kuning dengan bentuk bulat hingga oval dan pertumbuhannya dengan membentuk tunas.  Tujuannya adalah untuk mendapatkan isolat ini akan digunakan sebagai agen mikroba pada proses fermentasi menghasilkan etanol dari biomasa lignoselulosa sebagai bahan bakunya.   Berdasarkan morfologi menunjukan bahwa isolat-isolat ini adalah spesies Candida.  Isolat HT5 selanjutnya digunakan untuk melihat potensinya sebagai agen dalam produksi bioetanol.  Fermentasi menggunakan 5 g subtrat ampas tebu menghasilkan etanol  sebesar 0.0008%.  Hasil ini mengindikasikan bahwa isolat memiliki potensi sebagai agen fermentasi menghasilkan etanol akan tetapi masih perlu diteliti lebih lanjut untuk kondisi optimum prosesnya.

Kata kunci: spesies Candida, bioetanol. tuak

Downloads

Published

12/31/2016

Issue

Vol. 1 No. 3 (2016): Biodiversity

Section

Articles

License

The initial copyright of a paper is held by the authors (or their employer, in some instances). When publishing that paper the copyright may continue to be held by the author, or can be licensed or transferred to the publisher.

DOAJ recommends that journals allow authors to retain the copyright of their papers without restrictions. Authors will then grant the publisher the right of first publication, and other non-exclusive publishing rights.

Even when the author retains copyright, restrictions may sometimes be imposed by the publisher:

  • The publisher requires exclusive publishing rights. This means that the author no longer holds copyright without restrictions.
  • The publisher requires a transfer or exclusive license of commercial rights. This means the author no longer holds copyright without restrictions.

Submission of a manuscript implies that the submitted work has not previously been published (except as part of a thesis, report, or abstract); that it is not currently under consideration for publication elsewhere; and that its publication has been approved by all co-authors.

We need publishing rights from you in order to publish and make available an article. As a result, we require authors who publish in Sriwijaya journal of Environment to sign an author contract, which grants us the necessary publishing rights. This will occur after your manuscript has been peer-reviewed, accepted, and moved into production. Our Production team will then send you an email with all of the details. https://creativecommons.org/licenses/by-nc-sa/4.0/