Cholinetrase concentration in the blood of three types of cattle raised on land exposed to Organophosphates

Authors

  • Ali Harokan Universitas Sriwijaya Indonesia
  • Totong Kamaluddin
  • Daniel Saputra
  • Nurhayati Damiri

DOI:

https://doi.org/10.22135/sje.2021.6.1.67-72

Abstract

Organophosphate pesticides are one of the causes of contamination in animal feed. The cholinesterase enzyme test is used to diagnose toxic exposure due to organophosphate pesticides. Quantitative research with survey design using cross sectional design using 35 samples of cows. The study was conducted in March - September 2020. Concentration measurement on cholinesterase enzyme concentrations of blood plasma used the biosystem A15 DGKC-Colorimetric Kinetic method. This study aims to evaluate the concentration of the enzyme cholinesterase as a marker of organophosphate poisoning in cattle. Blood samples were collected from the ventrolateral neck veins of male and female cattle aged 1-7 years and collected from local cattle in the Ogan Ilir area. The results showed that the cows raised in breeders were exposed to organophosphate pesticides as indicated by a decrease in the concentration and levels of the cholinesterase enzyme in the plasma of female cows. Cholinestrase enzyme levels in female cows were 0.20 ± 0.067 U / ml and bulls were 0.27 ± 0.183 U / ml. The highest concentration of cholinesterase enzyme levels occurred in cows aged 2 years, namely 0.25 ± 0.34 U / ml and the lowest was in cows aged 7 years, namely 0.12 ± 0.04 U / ml.

Keywords:

Cows, Cholinesterase, Environment

References

. Kurniadi, D., & Maywita E.(2018). Factors Associated With Health Complaints Due to Pesticide Exposure to Horticultural Farmers in Siulak Deras Mudik Village, Kerinci Regency,Journal Menara Ilmu, XII,(80),13–8.

. Karabasanavar, N., Singh SP., Ahmad AH., Singh, S.K., & Rao, VDP.(2015). Monitoring of Animal Feed and Fodder Samples for Endosulphan Residues. Journal of veternary public health, 13,(2),119–22.

. Pardío, V., Martínez, D., Flores A., Romero, D., Suárez, V., López, K., & Uscanga,R.(2012). Human health risk of dietary intake of organochlorine pesticide residues in bovine meat and tissues from Veracruz, México. Journal Food Chemistry.135,(3),1873–93.

. Nag, S.K., & Raikwar, M.K (2010). Persistent organochlorine pesticide residues in animal feed. Journal Enviromental Monitoring and Assessment ,174 ,(1–4),327–35.

. Raini, M.(2007). Pesticide Toxicology and Treatment of Pesticide Poisoning. Research Media and Health Development. 17,(3),10–8.

. Glombowsky, P., Soldá, N. M., Campigotto, G., Volpato , A., Galli, G. M., Fávero, J. F., Bottari, N.B., Schetinger, M.R.R.C., Morsch,V.M., Baldissera,M. D., Machado,G., & Silva, A.S.(2017). Cholinesterase’s activities in cows supplemented with selenium, copper, phosphorus, potassium, and magnesium intramuscularly during the transition period. Journal Comparative Clinical Pathology.26,(3),575–9.

. Pardío, V.T., Ibarra, N., Rodríguez, M.A ., & Waliszewski, K.N. (2001).Use of cholinesterase activity in monitoring organophosphate pesticide exposure of cattle produced in tropical areas. Journal of Agricultural and Food Chemistry. 49,(12),6057–62.

. Abdelsalam, E.B., & Ford, E.J.H.(1985). Normal Esterase Activity in the Plasma, Whole Blood and Tissues of Cattle. Journal Zbl Vet Med A. 32,(1–10),518–25.

. Picco, E.J., Férnandez, H.R., Díaz David D.C., San André, M.I,, Boggio, J.C., & Rodríguez. C. (2008). Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration. Journal Environmental Science and Heathl - Part B Pesticides Food Contamunants and Agricultural Wastes. 43, (5), 405–9.

. Stepankova, S., & Komers, K.(2008). Cholinesterases and Cholinesterase Inhibitors. Journal Current Enzym Inhibition. 4(4) : 160–71.

. Mohammad, F.K., Alias, A.S., Faris ,G.A.M., & Al-Baggou, B.K .(2007). Application of an electrometric method for measurement of blood cholinesterase activities in sheep, goats and cattle treated with organophosphate insecticides. Journal Veterinary Medicine Series A Physiology Pathology Clinical Medicine. 54,(3),140–3.

. Cote, J.C., Lerman, J., & Anderson ,B.J.(2019). Phamacokinetics and pharmacology of drugs used in children. In: A practice of anesthesia for inflants and children (sixth Edition). ScinceDirect. p. 100–76.

. Colovic, M.B., Krstic, D. Z., Lazarevic-Pasti, T.D., Bondzic, A.M., & Vasic, V. M. (2013). Acetylcholinesterase Inhibitors. Pharmacology and Toxicology. Journal Current Neuropharmacology . 11(3) , 315–35.

. Lionetto, M.G., Caricato, R., Calisi, A., Giordano, M.E., & Schettino, T.(2013). Acetylcholinesterase as a biomarker in environmental and occupational medicine: New insights and future perspectives. Journal Biomed Research International. 2013. 1-8

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Published

05/01/2021

Issue

Vol. 6 No. 1 (2021): MANAGEMENT OF NATURAL AND MISCELLANEOUS RESOURCES

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